【摘要】 目的研究白细胞介素-6(IL-6)和白细胞介素-18(IL-18)在复发性流产(RSA)患者绒毛组织中的表达,探讨RSA的发病机制。方法采用实时荧光定量逆转录聚合酶链反应技术,检测63例RSA妇女(RSA组)及50例正常早孕妇女(正常组)绒毛组织中IL-6 mRNA和IL-18 mRNA的表达水平,并将两者进行相关性分析。结果 RSA组绒毛组织中IL-6 mRNA的表达水平显著低于正常组,IL-18 mRNA水平显著高于正常组,相关分析显示,RSA组IL-6与IL-18 mRNA两者之间表达水平呈负相关(r=-0.49,P<0.01)。IL-6 mR-NA的表达水平与RSA患者流产次数无关,而IL-18 mRNA表达水平随着流产次数增多而增加,差异有统计学意义(P<0.05)。结论正常妊娠较RSA患者绒毛组织IL-6水平高,当IL-6表达降低或IL-18升高时,可能导致RSA,其中IL-18水平与流产次数呈正相关。更多还原

【Abstract】 Objective To investigate the expression of interleukin-6(IL-6) and interleukin-18(IL-18) mRNA in villi of patients with recurrent spontaneous abortion(RSA) and to investigate the pathogenesis of RSA.Methods Real-time quantitative reverse transcription-polymerase chain reaction was used to quantify the expression of IL-6 mRNA and IL-18 mRNA in villi of 63 patients with RSA(RSA group) and 50 normal early pregnant women(normal group).The correlation between IL-6 and IL-18 was analyzed.Results IL-6 mRNA was significantly lower,while IL-18 mRNA was significantly higher,in RSA group than that in normal group.Meanwhile,there was significantly negative correlation between the IL-6 and IL-18 in RSA group(r=-0.49,P<0.01).Although there was no significant correlation between the number of abortion and IL-6 mRNA,IL-18 mRNA was significantly elevated with the increase of number of abortion(P<0.05).Conclusion The down-regulated IL-6 or up-regulated IL-18 can lead to RSA,as IL-18 is furthermore positively correlated with the number of abortion.更多还原