【摘要】 本研究以具有抗根肿病基因CRb的大白菜‘CR Shinki DH’系为供体亲本,大白菜自交系‘91-12B’为轮回亲本,通过回交和4个阶段的分子标记辅助选择,选育出7个携带CRb基因的抗根肿病近等基因系。通过CRb基因所在大白菜A3染色体的分子标记检测和抗病性鉴定,筛选出抗根肿病、并插入有供体亲本最短染色体片段的3个近等基因系B1S1、B2S2和B3S1。表型分析表明这3个近等基因系的结球相关性状与轮回亲本无显著差异,而且保持了‘91-12B’的配合力。所选育的近等基因系不仅能应用于育种实践,也为通过转录组分析研究根肿病的抗病机理提供了材料。更多还原

【Abstract】 The clubroot resistance gene CRb from donor parent’CR Shinki DH line’was introgressed into Chinese cabbage inbred line’91-12B’as recurrent parent by advanced backcross program and four-stage of marker-assisted selection.Totally,7 near isogenic lines(NILs) carrying the CRb gene were selected.Analysis of the 7 NILs with additional molecular markers distributed on A3 of Chinese cabbage chromosome,where CRb was located,and clubroot resistant assay,we found that 3 NILs,including B1S1,B2S2 and B3S1,contained the minimum genetic composition of donor parent,and showed resistance to clubroot disease.Phenotypic analysis of heading related traits indicated that there were no significant difference between these NILs and’91-12B’,but the combining ability was remained.These NILs might be useful for developing clubroot reistant cultivars in breeding program as well as for being a material to study the mechanism of the CRb gene though transcriptome analysis.更多还原