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水稻磷高效基因的初步定位

Molecular Mapping of Phosphorus-Efficient Gene in Rice

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【作者】 易俊良周德贵卢德城吴耀荣李宏黄道强赖穗春王重荣王志东陈立云谢旗周少川

【Author】 Yi Junliang 1,2* Zhou Degui 2* Lu Decheng 2 Wu Yaorong 3 Li Hong 2 Huang Daoqiang 2 Lai Suichun 2 Wang Chongrong 2 Wang Zhidong 2 Chen Liyun 1 Xie Qi 3** Zhou Shaochuan 1,2** 1 Rice Research Institute,Hunan Agricultural University,Changsha,410128;2 Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,510640;3 Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101

【机构】 湖南农业大学水稻科学研究所广东省农业科学院水稻科学研究所中国科学院遗传与发育生物学研究所

【摘要】 磷是植物生长发育所必需的大量营养元素。植物磷营养高效利用通常与根的形态、根分泌物、膜与体内磷转运以及菌根等因素有关,表现为受多基因控制。本文通过前期筛选工作,获得1份耐低磷株系。利用230对SSR和InDel引物,对低磷材料所在的回交导入系群体进行了初步定位,定位结果显示,与磷利用效率相关的基因座位有两个OsPe5和OsPe7,分别位于第5染色体的InDel520与InDel529标记之间,与InDel525标记共分离,物理距离约为900kb,和第7染色体InDel703与InDel717标记之间,与InDel713标记共分离,物理距离约为1400kb。

【Abstract】 Phosphorus(P) was an essential macronutrient for plant growth and development.High-efficient utilization of P-nutrition in plant was closely related to many factors,such as root morphology,root exudates,P-transportors across plasmalemma,P-translocation and symbiosis with microorganisms and so on.These nutritional properties were genetically controlled by multi-genes and characterized by quantitative trait loci(QTLs).In this study,a line with tolerance to low P was selected based on the identification of previous work.Backcross introgression lines(BIL) population with low-phosphorus materials were used for preliminary mapping by using 230 pairs of SSR and InDel primers.The results showed that two loci were associated with P utilization efficiency and mapped between the markers of InDel520 and InDel529 on the chromosome 5 and InDel703 and InDel717 on the chromosome 7,respectively.The locus on the chromosome 5 was co-segregated with the InDel525,about 900 kb physical distance,wher eas the locus on the chromosome 7 was co-segregated with the InDel713,approximately 1 400 kb physical distance.

【基金】 比尔和梅琳达·盖茨基金会项目(51587-13);农业部948项目(2006-G1)共同资助
  • 【文献出处】 分子植物育种 ,Molecular Plant Breeding , 编辑部邮箱 ,2012年06期
  • 【分类号】S511
  • 【被引频次】1
  • 【下载频次】265
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