【摘要】 本项研究以生产中用于杂交制种的8个色素万寿菊品种资源的花蕾期(Ⅰ)、鲜花半开期(Ⅱ)、鲜花盛开期(Ⅲ)的花瓣以及盛开期时的叶子(Ⅳ)为材料,采用实时荧光定量PCR方法,检测了叶黄素合成过程中3个相关基因lcyb(lycopene b-cyclase)、lcye(lycopene e-cyclase)和zds(ζ-carotene desaturase)在8个供试品种的不同发育时期的相对表达量。结果表明:3个基因的表达量在叶片中依次为lcyb>zds>lcye,花瓣中依次为lcye>zds>lcyb。lcye在花瓣中的表达量随花瓣的开放程度呈现先升高后降低的趋势,并在Ⅱ期达到最大值,且均高于叶片中lcye的表达量。Zds的表达趋势为Ⅱ>Ⅳ>Ⅰ>Ⅲ。Lcyb在叶中的表达高于花瓣中的任何时期。父本花瓣Ⅲ期的lcye表达量高于其他品种,且它的叶片中的lcyb的表达也高于其它品种。本项研究结果将有助于进一步阐明lcyb、lcye和zds基因与色素万寿菊叶黄素合成积累之间的关系,为进行色素万寿菊的基因工程育种奠定基础。更多还原

【Abstract】 In order to clarify the important roles of lcyb(lycopene b-cyclase),lcye(lycopene e-cyclase),zds(ζ-carotene desaturase) gene in the lutin biosynthetic pathway,real-time fluorescence quantitative PCR was employed to analyze the relative expression of these three genes in different tissues(bud,semi-open flower,fully open flower and leaf) of eight different kinds of pigment marigold cultivars.The results indicated that the relative expression level of these three genes should follow lcyb>zds>lcye in leaves,while lcye>zds>lcyb in petals.The relative expression level of lcye reached the maximum in semi-open flowers,whereas the relative expression trend of zds from high to low was in semi-open flower>leaf>bud>fully open flower.The relative expression level of lcyb in leaves was higher than in petals.The expression of lcye in fully open flower of paternal parent was also higher than that of other varieties.Results of this study would be helpful to further figure out the relationship between the genes of lcyb,lcye and zds and lutein synthesis and accumulation of pigment marigold,which might be a basis for the purpose of genetically engineered pigment marigold development.更多还原