【摘要】 建立了光度法测定硫普罗宁的新方法。研究表明:在SCN-和KNO3存在下,控制溶液pH4.0,Cu(II)被硫普罗宁还原生成的Cu(I)与SCN-反应形成CuSCN沉淀,该沉淀能浮在水相表面。通过测定溶液中剩余Cu(II)的量,可以测定硫普罗宁的含量。吸光度与硫普罗宁浓度之间存在良好线性关系。线性方程:A=4.898-0.3616ρ(μg/mL),线性范围为0.25～12.0μg/mL,相关系数R=0.9993,检出限为0.16μg/mL。该方法可直接用于药物中硫普罗宁含量的测定及其药代动力学行为研究。更多还原

【Abstract】 A novel spectrophotometric method has been developed to determine tiopronin in this paper.The study indicated that in the presence of SCN-and KNO3,Cu(Ⅱ) was reduced to Cu(Ⅰ) by the thiol group(-SH) of tiopronin and the resulting Cu(Ⅰ) was precipitated with SCN-to form the CuSCN precipitation that could be suspended in the upper part of water phase at pH 4.0.By determining the residual amount of copper(Ⅱ),the indirect determination of tiopronin can be achieved.Beer’s Law is obeyed in the range of 0.25-12.0 μg/mL of tiopronin at 510 nm with a linear correlation coefficient of 0.9993.The detection limit(LOD)(3 σ/k) is 0.16 mg/L.The method was successfully applied to the determination of tiopronin in pharmaceutical preparations and its pharmacokinetics.更多还原