【摘要】 将荧光染料分子标记的含29个碱基的可识别凝血酶的DNA适配体非特异吸附到纳米金表面,荧光发生猝灭,加入凝血酶后,凝血酶与适配体特异性结合,使适配体空间结构发生改变,荧光染料分子远离纳米金表面,荧光恢复,因此可以实现对凝血酶的检测。实验结果表明,这种检测方法简便、快速、特异性强,检出限为0.54 nmol/L(对应样品体积为200μL)。更多还原

【Abstract】 A 29-mer fluorophore-labeled aptamer against thrombin was adsorbed on gold nanoparticles(GNPs),causing fluorescence quenching.With the addition of thrombin,the binding of aptamer to thrombin would change the conformation of the aptamer,and the aptamer was released from GNPs,causing the recovery of fluorescence,so the detection of thrombin was achieved.Results showed this method was simple,fast and had good specificity.The detection limit was 0.54 nmol/L in 200 μL of solution.更多还原