【摘要】 根据阿米卡星、氢氯噻嗪在过氧化单硫酸盐(PMS)-鲁米诺(Luminol)体系中的化学发光反应的动力学性质有着明显的差异性,建立了同时测定阿米卡星和氢氯噻嗪新的时间分辨后化学发光的方法。在PMS-鲁米诺体系中,氢氯噻嗪化学发光反应较快,1 s达到最大值,峰尖锐;而阿米卡星化学发光反应较慢,37.9 s后达到最大值,峰平缓,且其动力学曲线呈现出随时间分开的两个独立的发光峰,互不干扰。结果表明;氢氯噻嗪和阿米卡星的线性范围分别为8.0×10-5～4.0×10-3g/L和8.0×10-4～4.0×10-2g/L;11次平行测定4.0×10-4g/L氢氯噻嗪溶液和4.0×10-3g/L阿米卡星溶液的化学发光强度相对标准偏差分别为2.4%和3.7%。本方法测定氢氯噻嗪和阿米卡星的检出限分别为2.0×10-5和2.0×10-4g/L。更多还原

【Abstract】 Based on the differential chemiluminescent rate of amikacin(AMK) and hydrochlorothiazide(HCT) in the system of Luminol-peroxymonosulfate(PMS),a novel time-resolved post-chemiluminescence method was established for the simultaneous determination of AMK and HCT.The HCT system gives the highest chemiluminescence intensity at 1 s,whereas the AMK system gives its most intense chemiluminescence emission at 39.7 s.The kinetic curve showed two separate chemiluminescence peaks at different time without disturbing each other.Under the optimum conditions described above,the analytical performance was obtained.The linear response for the determination of HCT and AMK was in the range from 8.0×10-5 to 4.0×10-3 g/L and from 8.0×10-4 to 4.0×10-2 g/L.The relative standard diviation of AMK(4.0×10-3 g/L) and HCT(4.0×10-4 g/L) are 3.7% and 2.4%(n=11),and the detection limit(3) of AMK and HCT are 2.0×10-4 g/L and 2.0×10-5 g/L,respectively.更多还原