【摘要】 采用基于核磁共振的代谢组学方法,分析了腹腔注射给药2,10和50 mg/kg体重剂量硝酸镨(Pr(NO3)3)168h内Wistar大鼠尿液和血清的核磁共振氢谱。由尿液及血清中内源性代谢物如柠檬酸、琥珀酸、α-酮戊二酸、肌酸酐、N-氧三甲胺、氨基酸、乳酸、牛磺酸及葡萄糖等的浓度变化,并结合大鼠血清指标研究了轻稀土化合物Pr(NO3)3在大鼠体内的急性生物效应。结果表明,Pr(NO3)3急性毒性的靶向器官为肝脏和肾脏,但以肝脏为主,且呈现明显的剂量-反应关系。低、中剂量组的Pr(NO3)3会通过改变大鼠体内酶代谢而造成肝脏线粒体中的能量代谢(脂肪、糖代谢)紊乱;同时,Pr(NO3)3还会影响肾脏的正常功能,改变肾脏中渗透质的平衡,影响肾脏对氨基酸的重吸收和利用。更多还原

【Abstract】 The acute biochemical effects of Pr(NO3)3 were studied by NMR-based metabonomic approach on rats urine and serum.Male Wistar rats were administrated intraperitoneally with Pr(NO3)3 at doses of 2,10 and 50 mg/kg body weight.Urine was collected over a 168 h period after dosing and serum was collected at 48,96 and 168 h period.1H NMR spectra of biofluids(urine and serum) were analyzed by principal components analysis.The results revealed that Pr(NO3)3 caused acute damages on rats liver and kidney,mainly in the liver,and showed a significant dose-dependent relationship.The liver and kidney injury induced by Pr(NO3)3 was characterized by the variations of many endogenous metabolites,such as citrate,succinate,α-ketoglutarate,creatinine,N-trimethyl dioxide,amino acids,lactate,taurine and glucose.更多还原