【摘要】 利用高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)联用技术对染砷后Chang肝细胞中的砷代谢产物进行分析。借助合成标准,采用标准加入法,表征出肝细胞中一种未知砷代谢产物甲基硫代砷酸(MMTA)。定量分析显示,MMTA含量随染砷浓度升高呈上升趋势。采用质量平衡分析法对双氧水处理前后的染砷细胞样品进行研究。结果显示,除小分子砷代谢产物外,细胞中部分砷代谢产物以大分子形态存在,推测其为砷蛋白类物质。由于MMTA系首次在人体肝细胞中发现,本研究结合实验现象和相关文献推测该物质系细胞代谢产物,并且其代谢途径与二甲基硫代砷酸相似,是以细胞内与其对应的一甲基亚砷酸为代谢起点。更多还原

【Abstract】 The hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry(HPLC-ICP-MS) was applied to the determination of arsenic metabolites in Chang liver cells after arsenic incubation.Monomethylthioarsonic acid(MMTA) was characterized by standard addition method using synthesized MMTA standard.Quantitative analysis showed that MMTA was increasing when initial incubated arsenic concentration increased.The results of mass balance approach suggested that some arsenicals were found as arsenic-protein bonded forms beside low molecular arsenical forms in hepatocytes.Furthermore,MMTA,which was first found in human hepatocytes,was speculated as a kind of metabolite after arsenic incubation.Like dimethylthioarsenicals,it derived from the metabolism of MMA(Ⅲ) in human hepatocytes.更多还原