【摘要】 目的应用红外显微光谱技术分析陈旧性心肌梗死组织中酰胺Ⅰ、Ⅱ、Ⅲ含量及相互关系,以探讨陈旧性心肌梗死过程中蛋白质分子的变化。方法选取20例冠心病猝死案例(均含陈旧性心肌梗死病变)的心脏样本,在每个心脏心肌梗死边缘处取材2块,常规固定、制作蜡块、连续切片2张,其中1张行HE染色,经Dotslide 2.1工作站扫描设定陈旧性心肌梗死区为B组(病变区),邻近非梗死病变区为A组(非病变区);另1张切片常规脱蜡、脱水,在扫描图像指导下,于相应区域行红外显微光谱微区检测,应用OMNIC 8.0工作站处理、分析光谱。结果与A组相比,B组显微光谱示酰胺Ⅰ、Ⅱ吸收峰的变化趋势一致,均为峰位蓝移、峰增宽、峰强度增加;酰胺Ⅲ吸收峰为峰位蓝移、峰增宽、峰强度降低;两组比较差异具有显著性意义(P<0.05,P<0.01);B组酰胺Ⅰ峰高/酰胺Ⅱ峰高增加(P<0.01)。结论与非病变区域心肌组织相比,陈旧性心肌梗死组织的蛋白质含量及构成均有差异,该病变过程存在氧化应激作用影响。更多还原

【Abstract】 Objective To analyze the contents of amide Ⅰ,Ⅱ,and Ⅲ,and their relevance in the tissues of old myocardial infarction using Fourier transform infrared micro-spectroscopy,and to reveal the changes of proteins during the pathologic process.Methods Myocardial samples were chosen from old myocardial infarction(OMI) areas in twenty cases died of sudden cardiac death.Two samples were taken from margin of the infarction region in each myocardial sample and were conventionally fixed,and embedded in paraffin.Two consecutive sections were prepared from each tissue block.One section was stained with H&E,and then scanned by Dotslide 2.1 workstation.The OMI region was defined as zone B(pathologic group),and the morphologically normal region adjacent to the OMI region was defined as zone A(non-pathologic group).Another section was measured by FTIRM in the relevant regions under guide of the scanned image.The spectra were processed and analyzed by OMNIC 8.0 workstation.Results As compared with zone A,changes of absorption bands for amide Ⅰ,and Ⅱrevealed similar tendency in zone B,including a blue shift in location,increase in width and intensity of the peaks.The band for amide Ⅲ showed a blue shift in location and increase in width,but decrease in intensity of the peaks.The differences between zone A and zone B were statistically significant(P<0.05,P<0.01).Meanwhile,the ratio of the intensity of amide Ⅰ to that of amide Ⅱ was increased in zone B(P<0.01).Conclusion It is suggested that the contents and components of proteins in the OMI tissues were different with those in the adjacent morphologically normal regions,which may be affected by oxidative stress in the pathologic process.更多还原