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CPP-ACP对牙骨质表面形貌及微区成分的影响
The Influence of CPP-ACP Upon the Root Surface and the Mineral Contents in Cementum
【摘要】 目的观察局部应用酪蛋白磷酸肽钙磷复合体(CPP-ACP)后牙骨质表面形貌的变化,并分析其微区成分,以明确CPP-ACP对牙骨质的影响。方法制备健康牙骨质块,以含10%CPP-ACP的GC Tooth Mousse分别涂擦21,14,7d,未涂擦组作为空白对照,并设标准对照组。扫描电镜观察各组牙骨质表面形貌,通过能谱分析测定牙骨质表面的微区成分(Ca、P含量及Ca/P),并进行比较。结果各组牙骨质表面形貌不同,与对照组相比,CPP-ACP处理14和21d后的牙骨质表面可见膜状覆盖物。各组牙骨质微区成分(Ca、P含量及Ca/P)的差别无统计学意义。结论含10%CPP-ACP的GC Tooth Mousse在健康牙骨质表面应用14d后,对牙骨质可能具有保护作用。
【Abstract】 Objective To observe the changes of root surface and analyze the mineral contents in cementum after local application of CPP-ACP,and to evaluate the effects of CPP-ACP paste on the cementum.Methods Cementum slices were obtained from healthy molars,then the slices were coated with GC Tooth Mousse(comtaining 10% CPP-ACP)for 21,14 and 7 days,respectivel.Control groups were set up.After the treatments,the surfaces were observed under field emission-scanning electron microscopy(FE-SEM).The SEM observations revealed different morphological features brought about by CPP-ACP,and the energy spectrometry analysis was used to measure the mineral contents in cenentum(Ca,P).Results There were obvious morphological differences between each group: a layer of membrane could be seen on the surface of cementum in the slices that were coated with GC Tooth Mousse for 14 and 21 days.The mineral contents of each groups were similar to that of control groups.Conclusion Local application of GC Tooth Mousse on healthy cementum after 14 days,a layer of member can been seen on the surface,and this member have protective effect on cementum.
【Key words】 Caseins,acid phosphatase; dental cementum; gas chromatography-mass spectrometry;
- 【文献出处】 福建医科大学学报 ,Journal of Fujian Medical University , 编辑部邮箱 ,2012年05期
- 【分类号】R781.05
- 【下载频次】99