【摘要】 目的探讨6-羟多巴胺(6-Hydroxydopamine,6-OHDA)诱导帕金森病大鼠模型脑中肿瘤坏死因子(tumor necrosis factorα,TNF-α)、白细胞介素-6(interleukin-6,IL-6)水平的改变。方法取SD大鼠80只,随机分成模型组与对照组,每组又分1,7,14,21d亚组。实验组大鼠左侧纹状体内注射6-OHDA,对照组注射等量生理盐水。术后各时间点观察各亚组经阿朴吗啡诱导的大鼠行为改变并行安乐死,取脑组织行免疫组织化学染色,观察黑质多巴胺能神经元数量变化;用酶联免疫吸附法测定大鼠中脑黑质组织匀浆中TNF-α、IL-6的水平。结果实验组大鼠术后7d出现不同程度的向健侧缓慢旋转(3.25±0.76)次/min,14d旋转次数增多(8.42±1.25)次/min,21d达到高峰(11.98±2.52)次/min;对照组各时间点均未发现向健侧旋转。实验组注射侧黑质TH阳性神经元数量1,7,14,21d组较对照组明显下降(P<0.01),且其数目的减少随时间延长而愈加显著,21d时下降到最低;实验组的TNF-α、IL-6水平较对照组持续增高。结论炎症因子TNF-α、IL-6参与6-OHDA制备帕金森病大鼠模型黑质多巴胺能神经元损伤过程。更多还原

【Abstract】 Objective To observe the changes of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) levels in rats with Parkinson disease(PD) induced by 6-OHDA.Methods 80 Sprague-Dawley(SD) rats were randomly divided into 1,7,14,21days experimental groups(n=10) and 1,7,14,21days control groups(n=10).6-OHDA was injected into the left striatum of brain in experimental groups,and normal saline was injected into the same target areas of brain in control groups.On each time point(1th,7th,14th,21th day respectively) after injection,both groups of rats induced by Apomorphine were observed and then euthanized.The number of tyrosine hydroxylase positive neurons in the substantia nigra(SN) was counted with immunocytochemical methods,and the expressive levels of TNF-α and IL-6 were detected with ELISA method.Results In experimental groups,the slight rotation behavior was observed on the 7th day after injection [(3.25±0.76) r/min],and the rotation behavior enhanced on the 14th day [(8.42±1.25) r/min] and peaked on the 21th day [(11.98±2.52) r/min],while there was no rotation behavior in the control groups.The tyrosine hydroxylase positive neurons in experimental groups decreased significantly on the 1th,7th,14th,21th day as compared with those of control groups(P<0.01),which decreased significantly along with time expansion,and reached the lowest on the 21th day.The expressive levels of TNF-α and IL-6 increased significantly on the 1th,7th,14th,21th day in experimental groups compared with those of control groups(P<0.01).Conclusion TNF-α and IL-6 probably participate in the occurrence and progress of 6-OHDA-induced PD in rats.更多还原