【摘要】 运用Real-time Q-PCR方法分析了Frizzed1-10mRNA在小鼠动情周期卵巢中的相对表达水平.结果发现10个Frizzed家族基因中,除Frizzed8外,其他均有不同水平的表达.其中Frizzed1和2在动情前期表达水平显著高于其他3个时期水平,约是间情期水平的4倍(P<0.05);动情前期和间情期的Frizzed3表达水平约是另2个时期的1.5倍(P<0.05);Frizzed4在动情期表达水平显著高于其他3个时期,约是间情期的3倍(P<0.05);间情期Frizzed5表达水平显著高于动情前期和动情期,约是动情期的5倍(P<0.05);Frizzed7表达类似于Frizzed5,间情期表达水平约是动情前期的4倍(P<0.05);Frizzed10的表达在动情前期表达水平约是动情期的3倍(P<0.05);Frizzed6和9在4个时期的表达无显著差别(P<0.05).以上结果表明:Frizzeds在4个动情时期卵巢中广泛而差异表达,并推测Frizzed1—4可能在动情周期卵泡发育过程中具有重要作用.更多还原

【Abstract】 Real-time Q-PCR was used to analyze the relative expression levels of Frizzed 1-10 mRNAs in the mice ovaries at different estrous stages.The results showed that they had different expression patterns except Frizzed 8.Expressions of Frizzed 1 and 2 were the highest in proestrus and both were about 4 times higher than those in diestrus(P<0.05).Frizzed 3 was 1.5 fold higher in proestrus and diestrus than those in the other two periods(P<0.05).Frizzed 4 was found significantly higher expression in estrus,which is about 3 fold than that in diestrus(P<0.05).Frizzed 5 was marked higher in diestrus than those in proestrus and estrus,being approximately 5 times higher than that in estrus(P<0.05).Frizzed 7 was similar to Frizzed 5,about 4 fold higher than the level in proestrus(P<0.05).Frizzed 10 expression in proestrus was about more than 3 times higher than that in estrus(P<0.05),but Frizzed 6 and 9 were no significant difference during the estrous cycle(P>0.05).These results suggested that Frizzeds were widely and differentially expressed in estrous ovary and Frizzed 1—4 may play a more important role in the development of follicle during the estrous cycle.更多还原