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发酵产生5-氨基乙酰丙酸的科氏葡萄球菌的筛选
Screening of Staphylococcus cohnii for Bioproduction of 5-aminolevulinic Acid
【摘要】 以发酵产生5-氨基乙酰丙酸(ALA)为目标,从自然界进行菌株的随机筛选,结果得到4株可生产5-氨基乙酰丙酸的细菌LA-2、LA-4、LA-7、LA-10。摇瓶发酵LA-10产量最高,达6.88mg.L-1。在常规生理生化特性测定基础上,结合16SrDNA序列分析,LA-10鉴定为科氏葡萄球菌(Staphylococcus cohnii),命名为科氏葡萄球菌LA-10。
【Abstract】 Four strains named LA-2,LA-4,LA-7 and LA-10 were distinguished by random screening of strains for bioproduction of 5-aminolevulinic acid.After shaking flask fermentation,the highest yield was 6.88 mg·L-1 from LA-10.The strain was identified as Staphylococcus cohnii based on the morphological,physiological,biochemical characteristics,and 16S rDNA analysis.It was named Staphylococcus cohnii LA-10.
【关键词】 5-氨基乙酰丙酸;
科氏葡萄球菌;
筛选;
发酵;
【Key words】 5-aminolevulinic acid; Staphylococcus cohnii; screening; fermentation;
【Key words】 5-aminolevulinic acid; Staphylococcus cohnii; screening; fermentation;
【基金】 福建省科技计划项目——省属公益类科研院所基本科研专项(2010R1025-4);福建省自然科学基金项目(2010J01106);福建省科技计划重大专项(2010NZ0002-3)
- 【文献出处】 福建农业学报 ,Fujian Journal of Agricultural Sciences , 编辑部邮箱 ,2012年09期
- 【分类号】TQ225.1
- 【被引频次】2
- 【下载频次】117