【摘要】 目的:用分散液液微萃取-气相色谱-质谱联用技术提取检测生物检材中的氯氮卓。方法:以氯仿为微萃取剂,甲醇为分散剂,在pH8提取尿样中的氯氮卓,气相色谱-质谱法分析,用内标标准曲线法测定氯氮卓的含量。结果:尿样中氯氮卓的线性范围为0.01～2μg/ml,检出限为0.002?g/ml;方法回收率为87.3%,RSD=3.47%(n=5)。结论:该方法操作简便、灵敏度高,适于尿样中氯氮卓的快速分析。更多还原

【Abstract】 Objective: To detect Chlordiazepoxide in urine by dispersive liquid-liquid micro-extraction and gas chromatography mass spectrometry.Methods: Chlordiazepoxide in urine was extracted with chloroform and methanol at pH8 and was analyzed by GC-MS,its content was determined with internal standard curve.Results: The detection limit of the Chlordiazepoxide in urine was 0.002 μg/ml,and the standard curve was linear in the range of 0.01 ～ 2 μg/ml.The average recovery of Chlordiazepoxide was 89.8%(RSD = 3.86%,n = 5).Conclusion: This method is simple,high sensitivity and suitable for quick analysis of the Chlordiazepoxide in urine.更多还原