【摘要】 目的探讨调肾祛斑颗粒(TSQBG)对黄褐斑的作用机理,为其临床应用提供科学依据。方法采用细胞学和血清药理学方法,观察TSQBG含药血清对长波紫外线(UVA)照射后的体外原代培养的人皮黑素细胞(MC)的细胞增殖的影响。结果不同浓度剂量TSQBG含药血清对UVA诱导的人皮MC的细胞增殖均有抑制作用,且浓度剂量越高抑制作用越强,并随作用时间的延长细胞增殖率不断降低,呈现量效和时效关系。结论 TSQBG能下调UVA诱导的人皮MC的细胞增殖率的上调,这可能是TSQBG治疗黄褐斑的重要作用机理之一。更多还原

【Abstract】 Objective In order to explore the treatment mechanism of TSQBG on Melasma,and to provide scientific basis for clinical application of TSQBG.Methods The experiment of the biological effects of TSQBG on melanocytes was done,cytology and seropharmacological methods were used,the intervention of TSQBG medicated serum on the cell proliferation of melanocytes after UVA radiation in vitro were observed,the primary cultured human skin melanocytes from foreskin were selected as target cells in this study.Results The different concentration of TSQBG has a potential effect on decreasing the cell proliferation of the primary cultured human skin melanocytes in vitro which increased by UVA.In addition,the intervention effects of TSQBG on the skin melanocytes after Ultraviolet A radiation was somewhat different from different treatment concentrations(doses) and time of TSQBG,the effect relative with the dosage,and stronger effect was showed in the middle,high concentration or dose group(P<0.05 or P<0.01),and were parallel with each other in a time-dependent manner.Conclusion The one important pathway of the biological effect of TSQBG on human skin melanocytes were decreasing the cell proliferation,and it is the possible mechanism of the effect of TSQBG on Melasma.更多还原