【摘要】 应用透射电镜和荧光显微镜对三种水生动物病毒粒子形态、病毒感染细胞的超微结构变化及亚细胞定位进行比较和研究。负染电镜观察显示:鳜鱼弹状病毒(SCRV)粒子呈典型的子弹状形态,长约76～118 nm,直径约29～52 nm;鲈鱼呼肠孤病毒(LJRV)粒子呈正二十面体结构,具有双层衣壳,直径约70～80 nm;蛙虹彩病毒(RGV)粒子呈正二十面体结构,具有囊膜,直径大小约150 nm。超薄切片观察表明,宿主细胞的基本结构遭到不同程度的破坏,成熟病毒粒子分布在胞质中或以出芽的方式释放到胞外,且RGV增殖后在胞质中聚集形成晶格状结构。免疫荧光观察进一步显示,RGV感染细胞后能产生多个直径大小不一的包涵体。本研究结果有助于了解和认识水生动物病毒的超微形态特征、复制过程及致病机理。更多还原

【Abstract】 Electron microscope and fluorescence microscope were used to observe and study three aquatic animal viruses,ultra-structural changes in infected cells and subcellular localization of viral proteins in fish cells.The negatively stained electron micrographs revealed that Siniperca chuatsi rhabdovirus(SCRV) has typical bullet-shape and the size ranges from 76 ~ 118 nm in length and 29 ~ 52 nm in diameter.Lateolabrax japonicus reovirus(LJRV) has icosahedral structure with a double-layered protein capsid,and the size is 70~80 nm in diameter.Rana grylio virus(RGV) is icosahedral in appearance with diameter of about 150 nm and composed of envelope.The electron micrographs of the ultra-thin section of infected cells suggested that the basic structures of infected cells had been damaged to a different extent,and the mature virus particles were distributed in the cytoplasm or released into the extra-cellular by budding.Moreover,paracrystalline arrays of RGV virus particles were observed in the cytoplasm of infected-cells.In addition,viral inclusion bodies were detected in RGV-infected cells by fluorescence assay.These results may help to understand the ultrastructural features,replication,and pathogenesis of different aquatic viruses.更多还原