【摘要】 为明确甘肃某牛场发生的疑似犊牛支原体肺炎的病原,利用支原体培养基从2份患肺炎的犊牛肺脏病料中分离到2个分离株,分别命名为GT-01和GT-02。通过菌落形态观察、生化试验、特异性PCR鉴定和16SrRNA测序比对对分离株进行鉴定。结果2个分离株在固体培养基上生长的菌落呈典型的"油煎蛋状",不能水解葡萄糖、甘露醇、精氨酸,不消化酪蛋白,PCR能扩增出牛支原体特异的1 911bp目的片段,16SrRNA基因序列与牛支原体国际标准株PG45的同源性分别为99.4%和99.6%。结果表明,2个分离株属于牛支原体。更多还原

【Abstract】 To identify the pathogen from calves showed pneumonia symptoms occurred in a cattle farm in Gansu province,two isolates(designated as GT-01 and GT-02) were isolated from two pneumonic lung tissue samples of calves using mycoplasma medium.Both isolates were characterized by the morphologic observation,biochemistry test,specific PCR test and sequence analysis of the 16 S rRNA gene.Colonies of two isolates grown on solid medium were typical fried-egg shape.Both isolates were unable to hydrolyze glucose,mannitol,arginine,and to digest casein.An expected gene fragment of 1 911 bp specific for Mycoplasma bovis were amplified by PCR.Sequence analysis of the 16 S rRNA gene revealed that GT-01 had an identity of 99.4 % and GT-02 had 99.6% identity with reference strain of PG45,respectively.The results indicated that the both isolates belonged to Mycoplasma bovis,which suggested that Mycoplasma bovis infection has been prevalent in some areas of Gansu province.更多还原