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以长白猪为受体母猪生产克隆五指山小型猪的妊娠诊断

Pregnancy Diagnosis of Landrace as Surrogate Sows Generating Cloned Wuzhishan Minipigs

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【作者】 王伟崔成哲李秀元王晓明尹熙俊李文学

【Author】 WANG Wei1,CUI Cheng-zhe2,LI Xiu-yuan2,WANG Xiao-ming2,YIN Xi-jun1,LI Wen-xue1(1.Agriculture College of Yanbian University,Yanji,Jilin,133002,China; 2.Yanbian Academy of Agricultural Sciences,Longjing,Jilin,133400,China)

【机构】 延边大学农学院延边农业科学院

【摘要】 采用B超结合猪早孕检测试剂盒对受体母猪进行妊娠诊断,确定了克隆五指山小型猪早期用B超进行妊娠诊断的最佳时间为胚胎移植后30d,可以检测到孕体和胎体反射;52d可以检测到胎儿的数量、胎儿骨骼及头部和脊柱;87d检测到胎动。在整个妊娠期采用B超联合猪早孕检测试剂盒进行妊娠诊断,能够及时排除干扰,提高克隆猪受体妊娠诊断的准确率。

【Abstract】 In this study,the real-time B-mode(brightness mode) ultrasonography associated with the early pregnancy diagnosis kit was used for pregnancy diagnosis in surrogate sows.The best time for early pregnancy diagnosis in cloned Wuzhishan minipigs was the 30th day after embryo transfer,when the gestational sac and carcass reflection can be detected;on the 52th day after embryo transfer,the number of fetuses,fetal bones,head and spine can be detected.Also on the 87th day the fetal movement can be detected.In addition,throughout the course of pregnancy,the use of real-time B-mode(brightness mode) ultrasonography associated with early pregnancy diagnosis kit for pregnancy diagnosis in surrogate sows can timely eliminate interference,improve the diagnostic accuracy of pregnancy in cloned Wuzhishan minipigs.

【关键词】 克隆猪胚母猪B超妊娠诊断
【Key words】 cloned pig embryosowB-mode ultrasonographypregnancy diagnosis
【基金】 国家转基因重大专项资金支持项目(2009ZX08006-002B)
  • 【文献出处】 动物医学进展 ,Progress in Veterinary Medicine , 编辑部邮箱 ,2012年10期
  • 【分类号】S858.28
  • 【被引频次】3
  • 【下载频次】65
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