【摘要】 目的分析20例胰腺癌及胰腺良性病变组织中miR-216a的表达差异,探讨miR-216a在胰腺癌中表达的临床意义及潜在应用价值。方法收集14例胰腺癌患者手术标本的癌组织,6例胰腺良性病变组织作为对照。采用Agilent人microRNA寡核苷酸基因芯片(V12.0)在基因组范围内检测20例组织标本的miR-216a表达,并分析其与胰腺癌临床病理参数的关系。结果胰腺癌组织中miR-216a的表达显著低于胰腺良性病变组织(P=0.000),miR-216a在胰腺癌组织中的表达与患者年龄、性别、吸烟、临床分期、局部淋巴结转移,远处转移、CA19-9浓度、肿瘤分化程度、神经束膜侵犯、脉管侵犯均无相关性(P>0.05),而与肿瘤T分期具有显著统计学意义(P=0.002)。结论 miR-216a在胰腺癌的发生、发展过程可能发挥重要作用,检测miR-216a有望成为胰腺癌新的肿瘤标记物或预后因子。更多还原

【Abstract】 Objective To explore the clinical significance of miRNA-216a expression in pancreatic cancer.Methods Fourteen patients with pancreatic cancer undergoing pancreticoduedenectomy and 6 patients with benign pancreas lesions were examined for miR-216a expressions in the tumor or lesion tissues using Agilent Human miRNA Microarray(V12.0).The relationship between miR-216a expressions and the clinicopathological features of the patients was analyzed.Results The expression of miRNA-216a was significantly lower in pancreatic cancer than in benign pancreas lesions(P=0.000).The expression of miRNA-216a was significantly correlated with the T stage of the tumor(P=0.002),but not with the patients’ age, gender,smoking status,tumor stage,lymph node metastases,distant metastasis,tumor differentiation,nerve invasion,vessel invasion or serum CA19-9 level(P>0.05).Conclusions The down-regulated expression of miR-216a in pancreatic cancer suggests the involvement of miR-216a in the tumorigenesis and development of pancreatic cancer.miR-216a may potentially serve as a novel rumor marker and also a prognostic factor for pancreatic cancer.更多还原