Myostatin(MSTN) is a member of the TGF-β superfamily that acts as a negative regulator of skeletal muscle growth.A full-length,2180 bp,cDNA sequence of the myostatin gene from Schizopygopisis pylzovi was cloned with RT-PCR,5'-RACE and 3'-RACE and the cDNA clone included a 1128 bp ORF,encoding a 375 amino acid peptide.Using PCR,we obtained the sequences of two introns of the MSTN gene and found that its structure in Schizopygopsis pylzovi was similar to that of other vertebrates,including three exons and two...
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