【摘要】 提出一种基于石蜡电极表面吸附孔雀石绿抗体的电化学免疫传感器,通过竞争型免疫检测法测量水体中残留孔雀石绿。对免疫传感器构制参数——电极表面预处理、抗-MG溶液pH、抗-MG溶液浓度和抗-MG抗体吸附时间进行了研究;对传感器测量过程参数进行了优化,传感器对孔雀石绿检测的线性范围为1.5～780μg/L,检测孔雀石的回收率在93%～116%。更多还原

【Abstract】 This paper proposes amperometric immunosensor based on the adsorptive immobilization of Malachite Green antibody on the solid paraffin electrode surface,measuring residues of Malachite Green in water bodies by competitive immuno-detected method.Meanwhile,it studies Fabrication parameters of the immune sensor(electrode surface pretreatment,pH of anti-MG solution,anti-MG solution concentration and anti-MG antibody adsorption time),optimizing parameters of sensor in measuring course,and obtaining 1.5～780 g/L of Malachite Green measuring range and 93%～116% of recovery rate.更多还原