【摘要】 以抗、感大豆胞囊线虫4号生理小种的灰皮支黑豆和晋豆11为材料,人工接种胞囊线虫,在出苗后定期取样,测定抗、感品种接种与未接种处理根系苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)活性以及根系内丙二醛(MDA)和超氧阴离子自由基(O.2-)含量的动态变化,以初步明确灰皮支黑豆抗大豆胞囊线虫4号生理小种的生化机制。结果表明,受大豆胞囊线虫4号生理小种侵染后,抗、感品种根系内PAL、SOD、POD、PPO酶活性均有增加,并且抗病品种根系酶活性增加相对较多,且持续时间长,而MDA和O.2-含量则在感病品种晋豆11中明显升高。在抗病品种根系中,接种前后MDA和O.2-含量变化不大。表明抗病品种比感病品种具有更强的防御能力和抗膜脂过氧化能力。更多还原

【Abstract】 In order to reveal the biochemical mechanism of Huipizhiheidou resistant to race 4 of Soybean Cyst Nematode,Huipizhiheidou and Jindou11 were used as resistant and susceptible varieties.The contents of phenylalanine ammonia lyase(PAL),superoxide dismutase(SOD),peroxidase(POD),polyphenoloxidase(PPO),malondialdehyde(MDA) and superoxide radicals(O-2 ·)were measured at 1,4,7,10,13,16,19,22,25,28,31,34,37 d after inoculation with cysts of SCN4.The results showed that the contents of PAL,SOD,POD and PPO in inoculated roots were higher than in non-inoculated roots.Compared to non-inoculated control,the increasing percentage of contents of PAL,SOD,POD,PPO in inoculated roots of Huipizhiheidou were more than those of Jindou11,and the fastigium in roots of Huipizhiheidou maintained longer time than in roots of Jindou11.After inoculation,contents of MDA andO-2 ·in roots of Jindou11 were higher than in roots of control plants.Contents of MDA andO-2 ·in roots of Huipizhiheidou maintained stable for inoculated and non-inoculated plants.These results implied that Huipizhiheidou has higher defense and antioxidant capacity than Jindou11.更多还原