【摘要】 以3个大豆品种为材料,采用GUS瞬时表达的方法,对适合于大豆子叶节和胚尖转化的基因型进行筛选,结果表明:适合大豆子叶节转化的品种为东农50,适合胚尖转化的品种为黑农41。在子叶节转化系统中,摸索了农杆菌侵染浓度与侵染时间的最佳配比组合、乙酰丁香酮浓度和超声波辅助处理对大豆转化效率的影响。优化后的条件为:农杆菌侵染的浓度OD600=0.6,侵染时间30～40 min,乙酰丁香酮浓度200μmol.L-1,超声波辅助转化时间5 s。在上述条件下,成功获得了转基因植株,转化效率为2.3%。更多还原

【Abstract】 Three soybean genotypes were used for optimal genotype selection in transformation mediated by Agrobacterium tumefacines.The influences of A.tumefacines concentration,infection time,acetosyringone(AS) concentration and sonication treatment were studied in this research.As a result,the appropriate genotype for cotyledonary node transformation was Dongnong 50 while Heinong 41 was fit for embryo tip transformation.In the cotyledonary node transform system,the optimal infection combination was A.tumefacines concentration at OD600=0.6 with 30-40 min infection time.The perfect AS concentration was 200 μmol·L-1.The appropriate sonicate treatment was 5 s under 100 W.The study optimized soybean cotyledonary-node Agrobacterium-mediated transformation system and transformation frequency was 2.3%.更多还原