【摘要】 用2,3环氧丙基三甲基氯化铵对壳聚糖进行改性,合成了两种不同取代度(DS:29%,48%)的壳聚糖季铵盐,通过FTIR、HNMR表征以证实其分子结构。用仿生共沉积法,在模拟体液中添加两种不同取代度的壳聚糖季铵盐,在镁合金AZ91表面修饰了一层薄的仿生涂层。SEM和XRD对涂层分析表明:经仿生修饰后的镁合金表面为含有钙磷盐/壳聚糖季铵盐的复合涂层,涂层晶体构型不同。抑菌实验结果表明:与含有钙磷盐/壳聚糖的复合涂层样品相比,含有钙磷盐/壳聚糖季铵盐的复合涂层对金黄色葡萄球菌具有明显的抑菌作用;比浊法测定显示:对金黄色葡萄球菌作用6 h,四种含有不同量的壳聚糖季铵盐样品均具有较好的抑菌效果,抑菌率为90%以上;作用24 h的结果表明,模拟体液中添加较低浓度的壳聚糖季铵盐制备的样品抑菌效果较好,抑菌率为98%。更多还原

【Abstract】 The quaternary ammonium salts of chitosan(HACC) were synthesized with two different degrees of substitution(DS:29%,48%) of quaternary ammonium by reacting chitosan with glycidyl trimethylammonium chloride.Characterization of HACC was analyzed with IR spectra and HNMR spectroscopy. A biomimetic coating was codeposited on the surface of AZ91 magnesium alloy by adding HACC of two DS into simulated body fluid(SBF).The CaP/HACC coatings were analyzed by scanning electron microscopy(SEM) and X ray diffraction(XRD).The coatings have different crystal types.The results of antibacterial assays show that the CaP/HACC coatings have more antimicrobial activity to Staphylococcus aureus than CaP/CS coatings.The absorbances at the 6th hour show that the bacterial inhibition rate of four samples exceeds 90%.The absorbances at the 24th hour show that the samples obtained by adding the HACC of lower concentration into simulated body fluid have better antimicrobial effect and their bacterial inhibition rate reaches 98%.更多还原