【摘要】 目的:探讨生长抑素类似物奥曲肽对胰腺癌细胞增殖、凋亡和血管内皮生长因子(VEGF)表达的影响。方法:分别加入不同浓度奥曲肽培养细胞24 h,采用四甲基偶氮唑蓝(MTT)法检测细胞增殖,原位末端转移酶标记法(TUNEL)检测细胞凋亡,实时荧光定量PCR(RT-PCR)法检测细胞VEGF mRNA表达。结果:随着奥曲肽浓度的升高,细胞增殖受到抑制,凋亡率升高,VEGFmNRA表达下降,与对照组比有显著性差异(P<0.05)。结论:奥曲肽可抑制胰腺癌细胞增殖、促进胰腺癌细胞凋亡和降低胰腺癌细胞VEGF的表达。更多还原

【Abstract】 Objective: To study the effects of somatostatin analogue Octreotide on proliferation、apoptosis and VEGF expression in human pancreatic cancer cell line.Methods: Proliferation was measured by MTT assay.Apoptosis was detected by TUNEL method.The expression of VEGF was analyzed by RT-PCR.Results: Octreotide inhibited the growth and VEGF expression of SW1990,and promoted the apoptosis in a dose-dependent manner(P<0.05).Conclusion: Octreotide may be used to clinical treatment by promoting apoptosis、inhibiting proliferation and VEGF expression in pancreatic cancer patient.更多还原