【摘要】 目的探讨普罗布考不同预处理疗程对大鼠硬化肝脏缺血再灌注(I/R)损伤保护作用及其机制。方法 30只Wist-ar雄性成年肝硬化大鼠随机分为5组(n=6),假手术组(A组)、对照组(B组)以及普罗布考不同预处理组(C、D、E组)。模型建立后测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)及碱性磷酸酶(AKP)的水平;测定肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、髓过氧化物酶(MPO)含量,并进行组织学观察。结果 I/R术前1d各组大鼠血清学检测指标无明显差异。再灌注2、4h,普罗布考不同预处理组血清转氨酶及肝组织SOD、MDA、MPO均优于B组(P<0.05)。在光镜下及电镜下观察各组肝组织及细胞微结构时,A组损伤最为轻,其次是E组,B组损伤最重。结论普罗布考对大鼠肝脏I/R的保护作用可能与增加内源性抗氧化剂、减少氧自由基及提高肝细胞抗缺氧能力等因素有关。更多还原

【Abstract】 Objective To explore the possible protective mechanism of preconditioning with different treatment courses of Probucol on liver ischemia/reperfusion(I/R) injury in rats.Methods A total of 30 male Wistar cirrhotic rats were randomly divided into five groups(n=6):sham-operation group(group A),control group(group B),groups of probucol preconditioning with terms(group C,D,E).The model had was made,then we determinated alanine aminotransferase,aspartate aminotransferase,lactate dehydrogenase and Alkaline Phosphatase in serum;Superoxide dismutase(SOD),Malondialdehyde(MDA),Myeloperoxidase(MPO) in tissue,While observing liver tissue and the cells.Results There was no significant difference in serological testing of the rats in each group the day before I/R.The groups of probucol preconditioning with terms were superior to the group B in serum transaminase and SOD,MDA,MPO of liver tissue at 2 h and 4 h after reperfusion(P<0.05).Observed each group of liver tissue and micro-structure of cells with light microscopy and transmission electron microscopy,group A was lightest injury of which,followed by group E,and the most serious injury was group B.Conclusion The mechanism of the pretreatment of Probucol may be related to the increasing level of endogenous antioxidants,reducing the release of oxygen free radicals and improving the ability of liver cells against hypoxia.更多还原