【摘要】 目的探讨2型糖尿病大鼠心肌血红素氧合酶-1(HO-1)不同表达水平与心肌氧化应激水平的关系。方法健康SD大鼠64只随机分为8组:空白6周(NC1)组、糖尿病6周(DM1)组、糖尿病加血红素6周(HE1)组、糖尿病加原卟啉锌(ZnPP)6周(ZN1)组;空白9周(NC2)组、糖尿病9周(DM2)组、糖尿病加血红素9周(HE2)组、糖尿病加原卟啉锌9周(ZN2)组,每组8只;分别在第6、9周处死大鼠,免疫组织化学法检测心肌HO-1表达水平,比色法检测心肌氧化应激水平,电子显微镜显示心脏超微结构改变。结果 (1)HE1、HE2组心肌HO-1的表达水平分别高于DM1、DM2组(P<0.05、P<0.05),显著高于NC1、NC2组(P<0.01、P<0.01)和ZN1、ZN2组(P<0.01、P<0.01)。(2)ZN1、ZN2心肌HO-1的表达水平低于NC1、NC2组(P<0.05、P<0.05);(3)HE1、HE2心肌纤维化程度和心肌线粒体超微结构损害明显低于DM1、DM2组和ZN1、ZN2组;(4)HE1、HE2组血清SOD和GSH-PX水平高于DM1、DM2组和ZN1、ZN2组(P<0.05,P<0.05;P<0.05,P<0.0 1)和(P<0.05,P<0.05;P<0.01,P<0.01),MDA水平低于DM1、DM2组和ZN1、ZN2组(P<0.01)而与NC1、NC2组比较,差异无统计学意义(P>0.05)。结论 (1)血红素可以提高心肌HO-1表达水平;(2)提高HO-1表达水平可增强2型糖尿病大鼠心肌抗氧化应激能力,减轻心肌损害。更多还原

【Abstract】 Objective To explore the relationship of the different level of HO-1 expression and the myocardial oxidative stress of type 2 diabetic rats.Methods 64 healthy SD rats were divided into eight groups randomly:six weeks blank group(NC1),six weeks diabetic group(DM1),six weeks diabetic+heme group(HE1),six weeks diabetic+ZnPP group(ZN1);nine weeks blank group(NC2),nine weeks diabetic group(DM2),nine weeks diabetic+heme group(HE2),nine weeks diabetic+ZnPP group(ZN2);each group contains eight rats and these rats would be killed in the sixth and ninth week to measure myocardial HO-1 express by IHCA;detect myocardial oxidative stress level by colorimetric method;display the change of myocardium ultra microstructure by electron microscope.Results(1) the expression level of HO-1 in group HE1,HE2 were higher than group DM1,DM2 respectively(P<0.05,P<0.05),and markedly higher than group NC1,NC2(P<0.01,P<0.01) and group ZN1,ZN2 respectively(P<0.01,P<0.01).(2)the expression level of HO-1 in group ZN1,ZN2 are lower than group NC1,NC2 respectively(P<0.05,P<0.05).(3) the degree of myocardium fibrosis and the ultrastructural damage of myocardium mitochondria in group HE1,HE2 were lighter than group DM1,DM2 and group ZN1,ZN2 respective ly.(4)the level of SOD,and SH-PX in group HE1,HE2 were higher than group DM1,DM2 and group ZN1,ZN2(P<0.05,P<0.0 5;P<0.05,P<0.01)and(P<0.05,P<0.05;P<0.0 1,P<0.01),the level of MDA were lower than group DM1,DM2 and group ZN1,ZN2(P<0.01) respectively but it was no statistical difference with group NC1,NC2.Conclusion(1)Heme can induce HO-1 express in myocardium.(2)Enhance the level of HO-1 can strengthen Protect Type 2 diabetic rats myocardium against oxidative stress.Thus it can alleviate pathological lesion.更多还原