【摘要】 目的分析淋巴瘤患者外周血中循环microRNA-21(miR-21)的表达,探讨循环miR-21在淋巴瘤早期诊断中的意义。方法收集60例临床确诊的淋巴瘤患者外周血,50名健康体检人群外周血作为正常对照组,用FQ-PCR方法检测miR-21的表达;同时检测对应的临床确诊淋巴瘤患者肿瘤组织及外周血中miR-21的表达,判断二者的相关性。结果 FQ-PCR检测结果显示,淋巴瘤患者外周血中循环miR-21的表达显著高于正常对照组,差异有统计学意义(t=6.9,P<0.000 1);且淋巴瘤患者外周血中循环miR-21的表达与肿瘤组织中miR-21的表达密切相关(r2=0.931,P<0.000 1)。结论外周血循环miR-21检测有望成为淋巴瘤早期诊断的新的分子标志。更多还原

【Abstract】 Objective To study the expression of serum circulating miR-21 in the patient with lymphoma and to validate its significance in early diagnosis of lymphoma.Methods We detected serum circulating miR-21 expression by FQ-PCR in lymphoma patients(n=60) and health controls(n=50).At the same time,we detected miR-21 expression by FQ-PCR in matched tumor tissue and serum from diffuse large B cell lymphoma Cymphoma patients(n=30).Results FQ-PCR results indicated miR-21 higher expression in serum from Cymphoma patients than controls(t=6.9,P<0.0001),and peripheral circulating miR-21 expression levels had very close correlation with those that matched tumor tissue in lymphoma cases(r2=0.931,P<0.0001).Conclusion Circulating miR-21 may be expected to be as a new biomarker to early detection of lymphoma.更多还原