【摘要】 目的:观察左归丸对D-半乳糖致衰老大鼠血液抗氧化能力和淋巴细胞DNA损伤的影响。方法:2月龄雄性SD大鼠30只,随机分为青年对照组、衰老组、左归丸组,每组10只。衰老组每日腹腔注射D-半乳糖400mg/kg,连续60d;青年对照组每日腹腔注射等体积0.9%氯化钠溶液;左归丸组处理同衰老组,同时按每日相当生药量4.97g/kg灌胃给药。采用黄嘌呤氧化酶法和硫代巴比妥酸(TBA)法检测血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;采用单细胞凝胶电泳(SCGE)检测淋巴细胞DNA损伤情况。结果:与青年对照组相比,衰老组大鼠血清SOD活性下降(P<0.01)、MDA含量升高(P<0.01),淋巴细胞DNA损伤增加(P<0.01);给予左归丸能显著提高D-半乳糖致衰老大鼠血清抗氧化能力,减少淋巴细胞DNA损伤。结论:左归丸可通过提高血液抗氧化能力、减少淋巴细胞DNA损伤而发挥其延缓衰老的作用。更多还原

【Abstract】 Objective: To investigate the effects of Zuogui Pill on blood anti-oxidative abilities and DNA damage of lymphocytes in aging rats induced by D-galactose.Methods: 30 male SD(2 months old) rats were equally divided into three groups randomly: young control group,old group,Zuogui Pill group.The rats in old group were administered D-galactose 400mg.kg-1.d-1 by intraperitoneal injection for 60 days consecutively and the rats in young control group were administered the equal volum normal sodium.And the intervention group were given Zuogui Pill with 4.97g.kg-1.d-1by intragastric administration at the same time.The SOD activity and MDA content in serum were measured.Single Cell Gel Electrophoresis(SCGE) was used to detect the DNA damage of lymphocytes.Results: Compared with young control group,the SOD activity were decreased and the content of MDA were increased in serum of the old group(P<0.01).The DNA damage of lymphocytes was increased(P<0.01).The variations above in aging rats were restored statistically in Zuogui Pill group.Conclusion: Zuogui Pill could slow down the aging progress through improving the blood anti-oxidative ability and decreasing the DNA damage of lymphocytes.更多还原