【摘要】 目的:探索中药复方"健脾活血方"中抗内毒素攻击作用的主效应中药及其主要药理作用环节点。方法:采用均匀设计法及尾静脉注射内毒素诱导的大鼠肝损伤模型,以血浆TNF-α含量为指标筛选主效应中药,并再次以该模型验证其药效;进一步检测肝组织CD68、CD14、TLR4、P-IκB蛋白表达及LBP、TLR2、TNF-α、IL-1β、IL-6的mRNA表达。结果:健脾活血方中抗内毒素攻击的主效应中药为枳壳、白芍、泽泻、五味子的组合;主效应中药可显著降低大鼠血浆TNF-α含量;主效应中药可显著降低大鼠肝组织CD68、CD14、TLR4、P-IκB蛋白表达,且其降低肝组织CD68蛋白有优于健脾活血方的优势;主效应中药可显著降低LBP、TLR2、TNF-α、IL-1β、IL-6mRNA表达,均有优于全方的优势。结论:枳壳、白芍、泽泻、五味子组合是健脾活血方中对抗内毒素诱发大鼠急性肝损伤的的主效应中药;主效应中药抗内毒素攻击的主要药理环节在于抑制肝脏库普弗细胞的活化。更多还原

【Abstract】 Objective: To investigate the major herbs in jianpihuoxue decoction against Lipopolysaccharide injury and find the action point of major herbs.Methods: The rats were injected in tail vein with Lipopolysaccharide to make the acute hepatic injury model.And uniform design was adopted to design the experiments.Portal vein plasma TNF-α was detected as the index.Screen out the major herbs and verify the efficacy with the same model.The protein expression of CD68,CD14,TLR4,P-IκB and the expression of LBP,TLR2,TNF-α,IL-1β,IL-6 mRNA in hepatic tissue were detected.Results: Fructus aurantii,White peony root,Alisma and Schisandra were the major herbs in jianpihuoxue decoction against Lipopolysaccharide injury.The major herbs could reduce the plasma TNF-α significantly.The major herbs could decrease the protein expression of CD68,CD14,TLR4,P-IκB,especially in decreasing CD68 which was better than Jianpihuoxue Decoction;LBP,TLR2,TNF-α,IL-1β,IL-6 mRNA expression in hepatic tissue were reduced apparently also,and the efficience of the major herbs was even better than Jianpihuoxue Decoction.Conclusion: The combination of Fructus aurantii,White peony root,Alisma and Schisandra were the major herbs of jianpihuoxue decoction against acute hepatic injury induced by Lipopolysaccharide.The action point of the major herbs was inhibiting the activation of kupffer cells.更多还原