【摘要】 目的探讨哮喘患者血清白细胞介素-25(IL-25)、白细胞介素-27(IL-27)在哮喘发展过程中的含量变化及其临床意义。方法分别选择64例急性加重期和临床缓解期哮喘患者,采用免疫散射比浊法测定血清中急性时相反应蛋白[铜兰蛋白(CER)、转铁蛋白(TRF)、α1-酸性糖蛋白(AAG)和结合珠蛋白(HP)]的浓度;ELISA法检测血清IL-25、IL-27浓度,并与30名健康对照者进行比较。并对急性加重期哮喘患者血清细胞因子IL-25、IL-27与急性时相反应蛋白(CER、TRF、AAG和HP)行相关性分析。结果哮喘患者临床缓解期组和急性加重期组HP、IL-25血清浓度均显著高于健康对照组(P<0.05),急性加重期较临床缓解组高(P<0.05);哮喘患者急性加重期TRF的含量显著低于健康对照组和临床缓解组(P<0.05),临床缓解期组TRF的含量出现上调,但与健康对照组比较无统计学意义(P>0.05);急性加重期哮喘患者血清IL-25、IL-27与急性时相反应蛋白(CER、TRF、AAG和HP)均无明显的相关性(P>0.05)。结论 HP能作为较好反映哮喘病情活动的指标,表明IL-25参与了哮喘的炎性病理过程,但其在哮喘炎性病理过程中起何具体作用有待进一步研究。更多还原

【Abstract】 Objective To investigate the change of serum interleukin-25(IL-25),interleukin-27(IL-27) and acute phase reaction protein(CER,TRF,AAG and HP) in patients with asthma of various types and explore its clinical significance.Methods The serum levels of CER,TRF,AAG and HP of 64 cases of asthma patients in acute exacerbation and clinical remission and 30 health controls were detected by immune scattering turbidimetry.The serum levels of IL-25,IL-27 were detected by ELISA.Results The serum IL-25 and HP levels in patients with clinical remission group and acute exacerbation were much higher than that of controls(P< 0.05),and the acute exacerbation group was much higher than clinical remission group(P<0.05).The levels of TRF in patients with asthma in acute exacerbation were significantly lower than those of healthy controls and clinical remission group(P<0.05).There was no significant difference of TRF between healthy controls and clinical remission group(P>0.05).There was no relationship between IL-25,IL-27,and acute-phase proteins(CER,TRF,AAG and HP) in the patients with asthma in acute exacerbation(P>0.05).Conclusion The results above indicate that HP can better reflect the activity of asthma,and IL-25 involve in the inflammatory pathogenesis of asthma.The specific role of IL-25 in inflammatory pathological process of asthma need to be further studied.Asthma;IL-25;IL-27;Acute phase reaction proteinIL-27,and acute-phase proteins(CER,TRF,AAG and HP) in the patients with asthma in acute exacerbation(P>0.05).Conclusion The results above indicate that H Pcan better reflect the activity of asthma,and IL-25 involve in the inflammatory pathogenesis of asthma.The specific role of IL-25 in inflammatory pathological process of asthma need to be further studied.更多还原