【摘要】 目的对比2种电化学酶联免疫检测体系检测人牙本质涎磷蛋白含量的差异性。方法选取人牙本质涎磷蛋白标准品以辣根过氧化物酶为标记酶,分别以邻联茴香胺(ODA)、邻苯二胺(OPD)为酶催化反应的底物,检测酶催化产物,对比两种检测体系下DSPP检测的线性范围及检测限的差异。结果 ODA-H2O2-HRP电化学酶联免疫检测体系与辣根过氧化物酶标记的双抗体夹心ELISA法相偶联,检测牙本质涎磷蛋白的线性范围为2.5～200.0 pg/ml,检测限为2.5 pg/ml,传统光度ELISA的检测限为5.0 pg/ml,灵敏度提高2倍;OPD-H2O2-HRP电化学酶联免疫检测体系检测DSPP的线性范围为1.0～200.0pg/ml,检测限为1.0pg/ml,比传统ELISA法的灵敏度提高5倍。结论 OPD-H2O2-HRP电化学酶联免疫检测体系较ODA-H2O2-HRP体系能更加精确地检测牙本质涎磷蛋白含量。更多还原

【Abstract】 Objective To compare the measurement accuracy of ODA-H2O2-HRP electrochemical enzyme-linked immunoassay system and OPD-H2O2-HRP electrochemical enzyme-linked immunoassay system in detecting dentin sialophosphoprotein(DSPP).Methods Standard DSPP was detected by ODA-H2O2-HRP electrochemical enzyme-linked immunoassay system,OPD-H2O2-HRP electrochemical enzyme-linked immunoassay system and traditional spectroscopic enzyme-linked immunosorbent assay respectively.The different results of the three methods were analyzed.Results By combining with the sandwich ELISA procedure,DSPP could be detected with OPD-H2O2-HRP electrochemical enzyme-linked immunoassay system in the concentration of 1.0 pg/ml to 200.0 pg/ml with the detection limit as 1.0 pg/ml,which was 5 times lower than spectroscopic ELISA method.DSPP could be detected with ODA-H2O2-HRP electrochemical enzyme-linked immunoassay system in the concentration of 2.5 pg/mL to 200.0 pg/mL with the detection limit as 2.5 pg/mL,which was 2 times lower than spectroscopic ELISA method.Conclusion The accuracy of OPD-H2O2-HRP electrochemical enzyme-linked immunoassay system in detecting standard DSPP is greater than that of ODA-H2O2-HRP electro che-enzyme-linked immunoassay system.更多还原