【摘要】 为探讨转化生长因子β1(TGF-β1)在蜕膜基质细胞中发挥免疫调节作用的机制,本研究以人妊娠初期的蜕膜基质细胞为研究对象,经0 ng/ml、1 ng/ml、5 ng/ml和10 ng/ml的TGF-β1处理后,运用RT-PCR方法检测趋化因子mRNA的表达,Western-blot检测趋化因子蛋白质的表达。结果表明:在mRNA水平和蛋白水平,高浓度的TGF-β1能够显著的下调蜕膜基质细胞中趋化因子配体CX3CL1、CXCL12和CXCL16的表达,有意义的上调趋化因子受体CXCR4和CXCR6的表达。研究结果提示,TGF-β1对趋化因子配体/受体有显著的调节作用,并通过趋化因子参与母胎界面的免疫调节。更多还原

【Abstract】 To explore the mechanism of immune regulation of transforming growth factor β1(TGF-β1) in human decidual stromal cells,the expression of chemokine in early human decidual stromal cells after treatment with four different concentrations of TGF-β1(0 ng/ml,1 ng/ml,5 ng/ml and 10 ng/ml) were detected by RT-PCR and Western blot.The results showed that high concentration of TGF-β1 significantly up-regulated the expression of chemokine ligand CX3CL1,CXCL12 and CXCL16,while down-regulated the expression of chemokine receptor CXCR4 and CXCR6 at the mRNA and protein levels.This study suggests that TGF-β1 plays a significant role in regulating chemokine ligand/receptor expression and may participate in immune tolerance of maternal-fetal interface by chemokines.更多还原