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I-45杨的离体培养

In vitro Culture of Populus × euramericana cv.I-45

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【Author】 ZHANG Ying1,MA Li-yuan1,ZHANG Gui-xue2,CUI Jian-guo1 (1.College of Forestry,Shenyang Agricultural University,Shenyang,Liaoning 110866;2.Shenyang Research Institute of Forestry and Pomology,Shenyang,Liaoning 110136)

【机构】沈阳农业大学林学院；沈阳市林业果树科学研究所；

【摘要】以I-45杨带腋芽的茎段为外植体,研究其离体培养中的最佳培养基和激素配比,建立I-45杨再生系统。结果表明:最佳诱导培养基为MS+KT 0.5mg/L+NAA 0.02mg/L,诱导率为82.2%;最佳继代培养基为MS+KT 1.0mg/L+NAA 0.02mg/L;诱导生根的培养基为1/2MS+IBA 0.05mg/L,生根率达86.7%。更多还原

【Abstract】 The suitable medium and plant growth regulator of plants were investigated by using popular axillary buds of nodal segments as explants.The results showed that MS medium supplemented with KT 0.5 mg/L and NAA 0.02 mg/L could induce shoots and the induction rate was 82.2%.Subcultured to MS medium added with KT 1.0 mg/L and NAA 0.02 mg/L could promote shoot growth.The rooting medium was 1/2MS medium in addition to IBA 0.05 mg/L,with the rooting rate of 86.7%.更多还原

【关键词】 I-45杨；离体培养；幼嫩茎段；植株再生；
【Key words】 Populus×euramericana cv.I-45； in vitro culture； tender stem segment； plant regeneration；

【基金】沈阳市科学技术计划资助项目(F10-137-9-00)

【文献出处】北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2012年05期

【分类号】S792.11
【下载频次】37

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