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苦瓜离体再生体系建立的研究

Study on the Establishment of in vitro Regeneration System in Momordica charontia L.

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【Author】 WU Peng1,FANG Feng-xue2,HUANG Ru-kui3,HUANG Xiong-juan3,CHEN Xiao-feng3(1.Guangxi Demonstration Park of Modern Agricultural Science and Technology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;2.Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;3.Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007)

【机构】广西农业科学院；广西现代农业科技示范园；广西农业科学院甘蔗研究所；广西农业科学院蔬菜研究所；

【摘要】以MS为基本培养基,附加不同浓度的激素组合进行苦瓜离体再生体系的研究。结果表明:种子去壳洗净浸泡5min,用70%酒精浸泡1min,洗净后0.1%HgCl2消毒10min的灭菌效果最好;通过愈伤组织诱导不定芽没有取得成功;利用无菌苗不同部位直接诱导不定芽,子叶节效果最好,最高诱导率达到100%,最适宜诱导培养基是MS+6-BA 2mg/L+IBA 0.02mg/L和MS+6-BA 4mg/L+IBA 0.01mg/L;最适生根培养基为1/2MS+IBA 0.2mg/L。更多还原

【Abstract】 In vitro regeneration system of Momordica charantia L.based on MS medium with various concentration of hormone combinations were studied.The results showed that the most effective disinfection was reached when the clean peeled seeds were socked for 5 min,70% ethanol for 1 min and rinsing before HgCl2 treatment for 10 min.Adventitious buds induction with callus was failed,while cotyledonary node reached 100% induction rate among all the parts of the sterile seedling and the optimum induction medium were MS+6-BA 2 mg/L+IBA 0.02 mg/L and MS+6-BA 4 mg/L+IBA 0.01 mg/L and the optimum root medium was 1/2MS+IBA 0.2 mg/L.更多还原

【关键词】苦瓜；离体培养；愈伤组织；不定芽；
【Key words】 Momordica charantia.L； in vitro culture； callus； adventitious bud；

【基金】广西农业科学院基本科研业务专项资助项目(200815(基))

【文献出处】北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2012年02期

【分类号】S642.5
【被引频次】8
【下载频次】151

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