【摘要】 目的:探讨紧密连接蛋白occludin的表达与人宫颈癌发生、发展、转移和预后的关系。方法:原位杂交检测occludinmRNA的表达;免疫组织化学和Western blot检测occludin的表达,并结合44例患者的临床资料进行分析。结果:occludinmRNA的表达在宫颈癌的大体类型和分化程度间差异均有统计学意义(P<0.05);occludin的表达在宫颈癌分化程度间差异有统计学意义(P<0.05);宫颈癌旁组织的occludin表达水平高于宫颈癌组织(P<0.05);宫颈癌组织与宫颈癌旁组织及正常宫颈组织的occludin表达水平差异均有统计学意义(P<0.01);随访满5年和满3年存活患者的occludin mRNA和满3年存活患者的occludin表达水平均高于病死患者(P<0.05)。结论:occludin在人宫颈癌组织中低表达,其表达与宫颈癌分化程度及患者生存期皆有一定关系,可作为宫颈癌诊断和预后判断的新指标。更多还原

【Abstract】 Objective:To investigate the relationship between the expression of occludin and the occurrence,development,metastasis and prognosis of human cervical cancer. Methods:The mRNA and protein expression of occludin were detected by in situ hybridization,immunohistochemistry and Western blotting,which combined with 44 patients clinical data were analyzed. Results:The occludin expression of different cervical cancer type and histologic differentiation was statistical different(P<0.05).The expression of occludin the adjacent tissues of cervical cancer tissue was higher than that of tumor tissues(P<0.05).The occludin expression levels in the adjacent tissues of cervical cancer tissue,tumor tissue and normal tissue were statistical different(P<0.01).The occludin mRNA expression levels of the survival patients with following up for 3 to 5 years were higher than that of the death patients(P<0.05). Conclusions:The expression of occludin is low in human cervical cancer,which has a positive correlation to the histologic differentiation of cervical cancer and survival time of patients and can be a new indicator for cervical cancer diagnosis and prognosis.更多还原