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3种检测方法在野鸟禽流感监测中的应用与比较
Application and Comparison of Three Methods in Avian Influenza Monitoring of Wild Birds
【摘要】 分别应用血凝抑制试验(HI)、反转录聚合酶链式反应(RT-PCR)与病毒分离3种检测方法对260份野鸟样品开展禽流感检测。结果表明,用HI方法检出禽流感样品57份,阳性检出率为21.92%;RT-PCR方法没有检出禽流感样品,阳性检出率为0;病毒分离方法检出禽流感样品15份,阳性检出率为5.77%。
【Abstract】 The 260 wild bird samples were detected by hemagglutination inhibition(HI),reverse transcription polymerase chain reaction(RT-PCR)and virus isolation.Results showed that the 57 samples were positive by HI test and the positive rate was 21.92%.No samples were positive by RT-PCR,and the positive rate was 0.The 15 samples were positive by virus isolation and the positive rate was 5.77%.
【关键词】 野鸟;
禽流感;
监测;
检测方法;
应用;
【Key words】 wild birds; avian influenza; monitoring; test methods; application;
【Key words】 wild birds; avian influenza; monitoring; test methods; application;
【基金】 新疆维吾尔自治区科技支疆项目(200991117)
- 【文献出处】 现代农业科技 ,Modern Agricultural Science and Technology , 编辑部邮箱 ,2012年15期
- 【分类号】S855.3
- 【下载频次】84