【摘要】 利用全自动氨基酸分析仪测定了传统保肝食品——河蚬汤中的总氨基酸和游离氨基酸含量。结果表明,河蚬汤中含有17种常见(色氨酸在酸解时遭破坏)氨基酸,还有鸟氨酸和牛磺酸两种非蛋白质组成氨基酸,总含量为297.4mg.g-1。其中鸟氨酸含量最高,占总氨基酸的12.4%,且87%的鸟氨酸以结合态形式存在。河蚬汤中游离氨基酸含量较低,为33.3mg.g-1,只占总氨基酸的11.2%。进一步分离、纯化河蚬汤中具生物学活性的蛋白和多肽,将有助于阐明其保肝有效成分及作用机理,为更好开发利用河蚬资源提供科学依据。更多还原

【Abstract】 Automatic amino acid analyzer was employed to investigate the total amino acid(TAA) and the free amino acid(FAA) of fresh clam soup,the most preferred folk medicine for liver protection and liver disease.Results showed that fresh clam soup contained 17 standard proteinogenic amino acids and two non-proteinogenic amino acids,ornithine and taurine.TAA content was 297.4 mg·g-1 while that was much lower of 33.3 mg·g-1 for FAA,accounting for 11.2% of TAA.Among all the amino acids,ornithine was of the highest count,12.4% of TAA,most of which may exist in the form of peptides.Further isolation and purification of bioactive protein and peptides in the soup will lead to clarification of the real liver protection ingredients and their mechanisms.Moreover,the results could provide scientific support for deep development of fresh clam industry.更多还原