【摘要】 目的建立马兰药材中大黄酸、大黄素及大黄酚含量的测定方法。方法采用高效液相色谱法(highperformance liquid chromatography,HPLC)。色谱柱:Kromasil-C18柱(250mm×4.6mm,5μm);流动相:甲醇-0.1%磷酸水溶液;梯度洗脱;检测波长:254nm;流速:1ml/min;柱温:30℃。结果大黄酸、大黄素、大黄酚浓度分别在0.253～2.530μg/ml(r=0.999 6)、0.249～2.490μg/ml(r=0.999 3)、0.257～2.570μg/ml(r=0.999 7)范围内与峰面积呈良好的线性关系;平均加样回收率(n=6)分别为99.27%(RSD=1.28%)、99.78%(RSD=1.05%)、99.92%(RSD=0.83%)。结论所建立的方法准确、可靠、简便,适合马兰药材中大黄酸、大黄素及大黄酚含量的测定。更多还原

【Abstract】 Objective To establish a method for determining the contents of rhein,emodin,and chrysophanol in Kalimeris indica by high performance liquid chromatography(HPLC).Methods HPLC was performed on Kromasil C18 column(250 mm×4.6 mm,5 μm) with a mobile phase of methanol-0.1% phosphoric acid for gradient elution at a detection wavelength of 254 nm,a flow rate of 1 ml/min,and a column temperature of 30℃.Results The concentrations of rhein,emodin,and chrysophanol showed a good linear relationship with peak areas in 0.253-2.530 μg/ml(r =0.9996),0.249-2.490 μg/ml(r=0.9993),and 0.257-2.570 μg/ml(r=0.9997),and the average recovery rates(n=6) were 99.27%(RSD=1.28%),99.78%(RSD=1.05%),and 99.92%(RSD=0.83%),respectively.Conclusion HPLC determination method can be used for measuring the contents of rhein,emodin,and chrysophanol in Kalimeris indica for its accuracy,reliability,and simplicity.更多还原