【摘要】 [目的]建立卡瓦胡椒(Piper methysticum)的离体再生体系。[方法]以卡瓦胡椒的带芽嫩茎和嫩叶作为外植体,通过在培养基中添加不同的激素组合,来筛选最佳再生培养基。[结果]卡瓦胡椒的丛芽诱导培养基为MS+4 mg/L 6-BA+0.5 mg/L GA3+0.65%琼脂+3%蔗糖+0.012 5%PVP,最佳芽伸长根生长培养基为1/2MS+5 nmol/L JA+0.65%琼脂+3%蔗糖。[结论]为卡瓦胡椒的工业化生产提供了理论指导。更多还原

【Abstract】 [Objective] This study aimed to establish in vitro regeneration system for Piper methysticum.[Method] With tender kava stems and leaves with bud as explants,different hormone combinations were added into basic medium,to screen the optimal regeneration medium.[Result] The optimal induction medium for multiple buds of kava was MS + 4 mg/L 6-BA + 0.5 mg/L GA3+0.65% agar + 3% sucrose + 0.012 5% PVP;the optimal regeneration medium for kava plantlets was 1/2MS +5 nmol/L JA +0.65% agar +3% sucrose.[Conclusion] This study provides theoretical guidance for the enlargement of industrial production of kava.更多还原