【摘要】 [目的]克隆马立克氏病鸡MDV血清基因,并对其进行鉴定。[方法]从潜伏期感染马立克氏病鸡淋巴组织中提取基因组DNA,采用梯度PCR扩增马立克氏病毒的L-meq基因。将其插入pMD18-T克隆载体并进行测序,并用DNAman软件进行序列分析。[结果]获得的DNA片段的序列与已发表的L-meq基因序列相一致,表明该试验成功获得了目的基因。[结论]该研究可为鸡马立克氏病的预防与治疗提供新思路和新方法。更多还原

【Abstract】 [Objective] To clone and identify Marek’s disease virus(MDV) serum gene.[Method] MDV genomic DNA was extracted from lymphoid tissues of the diseased chickens with latent MDV infection.The MDV L-meq gene was amplified by gradient PCR,inserted into pMD18-T vector and sequenced.The sequence was analyzed using DNAman software.[Result] The obtained sequence had 100% similarity with the published sequence of L-meq gene,showing successful amplification of target gene.[Conclusion] The paper provides new ideas and new methods for prevention and treatment of Marek’s disease in chickens.更多还原