【摘要】 目的:用L5178Y小鼠淋巴瘤细胞体外微核试验评价芦荟大黄素和芦荟提取物的诱变和抗诱变作用,为其安全性评价提供依据。方法:设溶剂对照、阳性对照和抗诱变对照,芦荟大黄素和芦荟提取物诱变和抗诱变试验各设4个剂量组,处理L5178Y细胞12 h后按常规方法进行体外微核试验分析。结果:较高浓度(6.67μg/ml)的芦荟大黄素可致微核细胞率增加,与对照组比较,差异有统计学意义(P<0.05);而芦荟提取物未见此效应。在一定剂量范围内,芦荟大黄素(0.22～6μg/ml)和芦荟提取物(20～180μg/ml)对甲磺酸甲酯(MMS)所致微核细胞率均有一定程度的拮抗作用,与对照组比较,差异有统计学意义(P<0.01)。结论:芦荟大黄素具有一定的诱变作用,而在本实验剂量范围内的芦荟提取物未见遗传毒性。两种受试物在一定范围内均能较好地拮抗MMS所致的染色体损伤。更多还原

【Abstract】 OBJECTIVE:To evaluate the mutagenicity and anti-mutagenicity of aloe-emodin and aloe extract by in vitro micronucleus test in L5178Y cells.METHODS:For 4 concentration of both aloe-emodin and aloe extract,mutagenicity and anti-mutagenicity groups were set up,as well as solvent control,positive control and anti-mutagenicity control.The L5178Y cells were treated respectively for 12 h,and in vitro micronucleus test was analyzed by routine method.RERULTS:Aloe-emodin showed mutagenic effect at 6.67μg/ml.Micronucleus rate at this dose showed a significant difference compared with the control group(P < 0.05),but aloe extract showed non-mutagenic effect in this study.Within a certain dose range,both aloe-emodin(0.22μg/ml) and aloe extract(20 |x g/ml) could exert antagonistic effect on the increase of micronucleus rate induced by MMS,showing a significant difference compared with the control group(P< 0.01).CONCLUSION:Under our experimental conditions,aloe-emodin showed weak mutagenic effect, while aloe extract was not found to have mutagenic effect.Both of them displayed anti-mutagenic activity.更多还原