【摘要】 目的:探讨不同类型淋巴瘤与EB病毒(Epstein-Barr virus,EBV)感染的关系。方法:收集淋巴瘤组织171例,包括弥漫大B细胞淋巴瘤(DLBC)106例;结外NK/T细胞淋巴瘤,鼻型22例;霍奇金淋巴瘤(HL)19例;血管免疫母细胞性T细胞淋巴瘤(AITL)13例;黏膜相关淋巴组织B细胞淋巴瘤(MALT)11例。应用EBV Lmp-1单抗免疫组化(IHC)和生物素标记的EBER1寡核苷酸探针原位杂交(ISH)分析EBV感染与淋巴瘤的关系。结果:淋巴瘤组织中EBV Lmp-1蛋白与EBER1 mRNA总阳性率分别为11.1%(19/171)、25.7%(44/171)。其中AITL为30.8%(4/13)、61.5%(8/13);HL为47.4%(9/19)、57.9%(11/19);结外NK/T细胞淋巴瘤为22.7%(5/22)、81.8%(18/22);DLBC为0.94%(1/106)、5.7%(6/106);MALT为0(0/11)、9.1%(1/11)。结果显示EBV在DLBC及MALT中的表达率低于AITL、HL及结外NK/T细胞淋巴瘤,差异有统计学意义(P<0.05);且原位杂交检测EBER1 mRNA比免疫组化检测Lmp-1蛋白更为敏感(P<0.01)。结论:EBV感染与淋巴瘤有密切关系,不同类型淋巴瘤与EBV感染的关系有差异。更多还原

【Abstract】 OBJECTIVE:To investigate the association between lymphomas and Epstein-Barr virus(EBV) infection.METHODS:We collected 171 lymphomas including diffuse large B-cell lymphoma(DLBC) 106 cases; extranodal NK/T cell nasal type lymphoma 22 cases;Hodgkin’s lymphoma(HL) 19 cases;angioimmunoblastic T-cell lymphoma(AITL) 13 cases and mucosal-associated lymphoid tissue(MALT) 11 cases.The expressions of EBV protein LMP-1 and EBER1 mRNA in 171 lymphomas were analyzed by immunohistochernistry(IHC) and in situ hybridization (ISH).RESULTS:The positive results of EBV-LMP-1 protein expression and EBER1 mRNA expression were 11.1%(19/171) and 25.7%(44/171) respectively,including 30.8%(4/13) and 61.5%(8/13) in AITL;47.4%(9/19) and 57.9%(11/19) in HL;22.7%(5/22) and 81.8%(18/22) in extranodal NK/T cell nasal type lymphoma;0.94%(1/106) and 5.7%(6/106) in DLBC.The expression rates of EBV in AITL,HL and nasal-type extranodal NK/T cell lymphoma were significantly higher than in MALT and DLBC(P<0.05).ISH was more sensitive than IHC in identification of EBV(P<0.01). CONCLUSION:There was a close association between EBV infection and lymphomas,with variation among different types of lymphomas.更多还原