【摘要】 目的探讨人参皂苷Rg1对骨髓间充质干细胞(mesenchymal stem cell,BMSCs)移植后阿尔茨海默病(Alzheimer′s disease,AD)大鼠学习记忆能力的影响及机制。方法雄性SD大鼠75只,采用随机数字表法分为双侧穹窿海马伞(hipp-ocampal fimbria-farnix,FF)切断模型组(模型组),假手术组(除不切断FF以外,其他操作同模型组),人参皂苷Rg1治疗组(Rg1组:造模后2周给予腹腔注射人参皂苷Rg1,每天1次,共4周),BMSCs移植治疗组(BMSCs组:造模后2周,每只大鼠接受1×106个BMSCs治疗)。人参皂苷Rg1联合BMSCs治疗组(联合组:人参皂苷Rg1在BMSCs移植治疗同时给药)。采用Morris水迷宫检测各组动物逃避潜伏期(s),RT-PCR检测基底前脑神经生长因子(NGF)mRNA表达。结果双侧FF切断术后6周,各用药组与模型组比较,逃避潜伏期缩短,提示痴呆大鼠学习记忆能力有所改善;联合组大鼠学习记忆能力、NGF mRNA平均表达水平分别为29.95±2.03、1.13±0.15,BMSCs组分别为44.36±1.43、0.78±0.09,联合组优于BMSCs组(P<0.05)。结论人参皂苷Rg1能够加强BMSCs移植治疗AD模型大鼠的学习记忆能力,其机制可能与人参皂苷Rg1上调BMSCs植入痴呆大鼠基底前脑神经元NGF的表达有关。更多还原

【Abstract】 Objective To study the effect and mechanism of ginsenoside Rg1 on the spatial learning-memory ability in rats with Alzheimer′s disease after transplanted with bone marrow mesenchymal stem cells(BMSCs).MethodsUsing digital randomization table method,seventy-five male SD rats were divided into the bilateral FF transection model group(as the model group: ambi-hippocampal fimbria-fornix transected),the sham-operative control group(the SOC group: receiving the same modeling process as the model group,but without ambi-hippocampal fimbria-fornix transected),the ginsenoside Rg1 treatment group(as the treatment group: Two weeks after modeling ginsenoside Rg1 was peritoneally injected at the dose of 5 mg/kg,once daily for four weeks in total),the BMSCs transplanted treatment group [as the control group: Two weeks after modeling every rat received transplantation of BMSCs(10 μL,1×106 cells)],and the ginsenoside Rg1+BMSCs treatment group(as the combination group: They received both transplantation of BMSCs and peritoneal injection of ginsenoside Rg1).The spatial learning-memory ability of rats was detected by Morris water maze and the escape latency(s) was recorded.mRNA expression of nerve growth factor(NGF) was detected using Real-time PCR.Results Six weeks after the hippocampal fimbria-fornix(FF) transection,the escape latency of each medication group was obviously shorter than that of the model group,and the spatial learning-memory ability of dementia rats was somewhat improved.The spatial learning-memory ability of rats in the combination group was(29.95±2.03) and the mRNA expression level of NGF was(1.13±0.15),better than those in the BMSCs group(44.36±1.43,0.78±0.09,P<0.05).Conclusions Ginsenoside Rg1 could strengthen the spatial learning-memory ability in dementia rats after transplanted with BMSCs.Its mechanism might be possibly correlated with up-regulating mRNA expression of NGF in basal forebrain after BMSCs transplantation.更多还原