【摘要】 目的:探讨黑素瘤抗原(melanoma antigen,MAGE)-A11在乳腺癌组织及相应癌旁组织中的表达,以及MAGE-A11对乳腺癌细胞增殖的影响。方法:应用免疫组织化学法检测60例乳腺癌组织及其相应癌旁组织中MAGE-A11蛋白的表达,分析其与乳腺癌患者临床病理学特征之间的关系。将pCMV-AC-MAGE-A11-GFP表达质粒转染人乳腺癌MCF-7细胞后,应用MTT法及集落形成实验检测MAGE-A11对乳腺癌细胞增殖的影响。结果:60例乳腺癌组织中有31例MAGE-A11蛋白阳性表达,阳性率为51.7%,相应的癌旁组织中均未发现MAGE-A11蛋白的表达;MAGE-A11蛋白的表达与乳腺癌患者的年龄、病理类型、组织学分级、临床分期、肿瘤大小、淋巴转移、雌激素受体(estrogen receptor,ER)-α、孕激素受体(progestrogen receptor,PR)及乳腺癌扩增因子1(ampli?ed in breast cancer1,AIB-1)的表达均无明显关系(P>0.05),与人表皮生长因子受体2(human epidermal growth factor receptor2,HER-2)/C-erbB2和ER-β的表达有关(P<0.05)。转染pCMV-AC-MAGE-A11-GFP表达质粒后,MCF-7细胞的增殖率和集落形成数均有明显增加(P<0.05)。结论:乳腺癌组织中MAGE-A11蛋白的表达与C-erbB2和ER-β的表达有关,且MAGE-A11可促进人乳腺癌MCF-7细胞的增殖。更多还原

【Abstract】 Objective:To investigate the expression of melanoma antigen(MAGE)-A11 in breast cancer tissues and their corresponding para-cancerous tissues and the effect of MAGE-A11 expression on the proliferation of breast cancer cells.Methods:The expression of MAGE-A11 protein in breast cancer tissues and their corresponding para-cancerous tissues from 60 cases was detected by immunohistochemisty.The relationship between the expression of MAGE-A11 protein and the clinicopathological features was analyzed.Human breast cancer MCF-7 cells were transfected with pCMV-AC-MAGE-A11-GFP plasmid.The effect of MAGE-A11 expression on the proliferation of breast cancer cells was detected by MTT method and colony formation assay.Results:Of 60 samples of breast cancer tissue,31 had positive expression of MAGE-A11,and the positive expression rate was 51.7%.The expression of MAGE-A11 protein was negative in 60 samples of para-cancerous tissue.The expression of MAGE-A11 protein was not related to the age,pathological type,histological grade,clinical stage,tumor size,lymph node metastasis,estrogen receptor(ER)-α,progestrogen receptor(PR),and amplified in breast cancer 1(AIB-1) in patients with breast cancer(P>0.05),while the human epidermal growth factor receptor 2(HER-2)/C-erbB2 and ER-β were related to the expression of MAGE-A11 protein(P<0.05).The proliferation rate and the number of colony-forming units of MCF-7 cells were significantly increased after transfection with pCMV-AC-MAGE-A11-GFP plasmid(P<0.05).Conclusion:The expression of MAGE-A11 protein is related to the expressions of C-erbB2 and ER-β in breast cancer tissues.MAGE-A11 may promote the proliferation of human breast cancer MCF-7 cells.更多还原