The unsaponifiable matter was extracted from saponified sesame oil by petroleum ether,and it was dissolved and diluted with methanol to volume.After the above treatment,the sesamin in sesame oil was quantitatively determined by high performance liquid chromatography(HPLC).A suitable determination conditions were as follows : GL Sciences column(ODS-3,250 mm×4.6 mm,5 μm),mobile phase methanol-water(volume ratio 80∶ 20),flow rate 1 mL/min,detecting wavelength 287 nm,column temperature 40 ℃.The retention time o...