【摘要】 目的比较心包部位和腹股沟部位脂肪组织来源干细胞(ADSC)的免疫表型和分化潜能。方法取Wistar大鼠心包组织和腹股沟脂肪,消化分离原代ADSC,分别用流式细胞技术和免疫组织化学法检测两个部位ADSC的免疫表型和心肌源性转录因子的表达,并进一步用5-氮杂胞苷分别诱导两个部位的ADSC向心肌细胞分化,分析诱导后心脏肌钙蛋白T(cTnT)的表达。结果两个部位的ADSC具有相同的免疫表型:均为CD29、CD44、CD90阳性和CD34、CD45阴性细胞。原代心包部位的ADSC可以检测到少量cTnT阳性细胞,并有不同程度的GATA-4、Isl-1、Nkx-2.5和MEF-2c表达;而原代腹股沟部位的ADSC除了Isl-1呈弱阳性表达外,其他因子均为阴性表达。两个部位的ADSC经诱导均定向分化成心肌细胞,但心包部位的ADSC的心肌分化能力大于腹股沟部位的ADSC(53%与32%,P<0.01)。结论不同部位的ADSC有共同的形态和免疫表型。心包部位的ADSC在转录因子和cTnT表达方面体现出较强的心肌分化潜能。更多还原

【Abstract】 Objective To compare the immune phenotype and the myogenic potential of 2 adipose tissue-derived stem cell(ADSC) populations from the pericardial tissue and the groin in rats.Methods The 2 ADSC populations were cultured from the pericardial tissue and the groin tissue in Wistar rats.The immune phenotypes of the 2 ADSC populations were evaluated by flow cytometry and the expression of myocardium-derived transcription factors was assessed by immunohistochemistry.After ADSC being induced to cardiomyocytes,the expression of cardiac troponin T(cTnT) was detected in 2 ADSC groups.Results Flow cytometry analysis showed that the 2 ADSC populations shared identical immune phenotypes,i.e.positive for CD29,CD44 and CD90,but negative for CD34 and CD45.However,the pericardial ADSC showed a various extent expression of GATA-4(62%±19%),Isl-1(41%±13%),Nkx-2.5(4.3%±2%),and MEF-2c(32%±11%).Most interestingly,the pericardial ADSC exhibited greater potential of myogenic differentiation,marked by the expression of cTnT,than the groin derived ADSC(53% vs 32%,P<0.01).Conclusion Pericardial ADSC shared biologically comparable properties to cardiac stem cells and represented an attracting cell source for cardiac cellular therapy.更多还原