【摘要】 目的探讨芹菜素对缺血再灌注大鼠心肌细胞凋亡相关基因Bcl-2/Bax表达的影响。方法心肌缺血45 min,再灌注2 h制备缺血再灌注模型。随机分为正常组、假手术组、生理盐水缺血再灌注组、溶剂对照组、美托洛尔阳性对照组及芹菜素低、中、高剂量组,每组8只。除假手术组外,余各组在缺血前10 min分别股静脉给药。再灌注2 h后迅速取出心脏,用RT-PCR测Bcl-2及Bax基因的mRNA表达;实验时记录心率(HR)、平均动脉压(MAP)、心电图S-T段并计算心律失常Lambeth评分。结果芹菜素组与生理盐水缺血再灌注组比较,Bcl-2 mRNA表达量增加,Bax mRNA表达量减少,均呈剂量依赖性(P<0.05);心律失常Lambeth评分芹菜素组低于假手术组(P<0.05);芹菜素能缩短再灌注心律失常持续时间(P<0.05)。结论芹菜素对大鼠心肌缺血再灌注损伤具有良好的保护作用;芹菜素抗心肌凋亡作用的机制可能与其上调Bcl-2基因表达和下调Bax基因表达有关。更多还原

【Abstract】 Objective To investigate the effects of apigenin on expressions of apoptosis-related genes Bcl-2/Bax in cardiomyocyte of rats with myocardial ischemia and reperfusion.Methods Sixty-four rats were randomly divided into eight groups:Normal group,sham-operation group,physiological saline ischemia-reperfusion group(NS group),solvent control group(Sol group),metoprolol positive control group(Meto group),apigenin low,mid and high dose group(Api1,Api2,Api4).The rats were treated for 10 min before ischemia except rats in sham-operation group.The rat models with ischemia-reperfusion were established with 45 min of myocardial ischemia,2 h reperfusion.Then the heart was quickly removed.The expression levels of Bcl-2 mRNA and Bax mRNA were detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Heart rate(HR),mean arterial pressure(MAP),and electrocardiogram(ECG) ST segment were recorded and the Lambeth arrhythmia score was calculated.Results Compared with NS group,the expression of Bcl-2 mRNA was dose-dependently increased,and the expression of Bax mRNA was dose-dependently reduced in apigenin groups(P<0.05).The Lambeth arrhythmia score in apigenin groups was significantly lower than that in control group(P<0.05).The duration of reperfusion arrhythmia was shorten in apigenin groups(P<0.05).Conclusion Apigenin had a protective effect on rats with myocardial ischemia and reperfusion.The apigenin could up-regulate the expression of Bcl-2 and down-regulate the expression of Bax at transcription level,which could be related to anti-apoptotic effect.更多还原