【摘要】 目的:研究隔山消及其不同炮制品的抗炎作用及机制。方法:采用二甲苯致小鼠耳廓肿胀、角叉菜胶致小鼠足肿胀和醋酸致小鼠腹腔毛细血管通透性试验观察其抗炎作用,测定对炎症渗出液中前列腺素E2(PGE2)以及血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)和一氧化氮(NO)的影响。结果:隔山消生品及炮制品(24、和8g/kg)对小鼠耳廓肿胀及足肿胀均有明显的抑制作用,并可显著抑制醋酸致小鼠腹腔毛细血通透性增加。能够明显降低炎症渗出液中PGE2和血清中MDA、NO含量,明显提高小鼠血清SOD和CAT活力。结论:隔山消及两种炮制品均有一定的抗炎作用,两种炮制品的抗炎效果比生品有所增强。抗炎作用机制可能与其抑制PGE2、NO等炎症介质生成,抑制脂质过氧化、增强自由基清除能力有关。更多还原

【Abstract】 Objective:To study anti-inflammatory effects and mechanisms of Cynanchumau ricutalum Royle ex Wight.and different processed products.Methods:Dimethyl benzene-induced auricular swelling model,carrageen-induced metatarsal swelling model and acetic acid-induced abdominal capillary permeability increase in mice were established to observe the anti-inflammatory effects.The model of metatarsal swelling induced by carrageenin was used to determine the effects of on the content of prostaglandin E2(PGE2) in the exudates and SOD,MDA,CAT,NO in blood serum.Results :Cynanchumau ricutalum Royle ex Wight.and different processed products(2、4 and 8g/kg) could significantly inhibit auricular swelling induced by dimethyl benzene,metatarsal swelling induced by carrageenin of mice,inhibit abdominal capillary permeability,reduce PGE2,MDA,NO contents in blood serum and increase CAT,SOD activity in blood serum.Conclusion :Crude and processed products have significantly anti-inflammatory effects and the effects of processed products is stronger than crude.The mechanisms involved in the anti-inflammatory are related to the decrease of abdominal capillary permeability,reduction of inflammatory mediators and augmentation of oxygen free radical scavenging capacity and anti-lipid peroxidation.更多还原